High-throughput genetic manipulation of multicellular organisms using a machine-vision guided embryonic microinjection robot.

ABSTRACT: Microinjection is a technique used for transgenesis, mutagenesis, cell labeling, cryopreservation, and in vitro fertilization in multiple single and multicellular organisms. Microinjection requires specialized skills and involves rate-limiting and labor-intensive preparatory steps. Here, we constructed a machine-vision guided generalized robot that fully automates the process of microinjection in fruit fly (Drosophila melanogaster) and zebrafish (Danio rerio) embryos. The robot uses machine learning models trained to detect embryos in images of agar plates and identify specific anatomical locations within each embryo in 3D space using dual view microscopes. The robot then serially performs a microinjection in each detected embryo. We constructed and used three such robots to automatically

SUBMITTER: Alegria AD 

PROVIDER: S-EPMC10990426 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature