Gut microbiota in vaccine naive Gabonese children with rotavirus A gastroenteritis.

ABSTRACT:

Background

While the gut microbiome modulates the pathogenesis of enteric viruses, how infections caused by rotavirus A (RVA), with or without diarrhoea, alter the gut microbiota has been sparsely studied.

Methods

From a cohort of 224 vaccine naïve Gabonese children with and without diarrhoea (n = 177 and n = 67, respectively), 48 stool samples were analysed: (i) RVA with diarrhoea (n = 12); (ii) RVA without diarrhoea (n = 12); (iii) diarrhoea without RVA (n = 12); (iv) healthy controls without diarrhoea and RVA (n = 12). The 16S rRNA metabarcoding using Oxford Nanopore sequencing data was analysed for taxonomic composition, abundance, alpha and beta diversity, and metabolic pathways.

Findings

Alpha diversity showed that children with acute diarrhoea (with and without

SUBMITTER: Manouana GP 

PROVIDER: S-EPMC10990958 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature