Identification of a chromatin-bound ERRα interactome network in mouse liver.

ABSTRACT:

Objectives

Estrogen-related-receptor α (ERRα) plays a critical role in the transcriptional regulation of cellular bioenergetics and metabolism, and perturbations in its activity have been associated with metabolic diseases. While several coactivators and corepressors of ERRα have been identified to date, a knowledge gap remains in understanding the extent to which ERRα cooperates with coregulators in the control of gene expression. Herein, we mapped the primary chromatin-bound ERRα interactome in mouse liver.

Methods

RIME (Rapid Immuno-precipitation Mass spectrometry of Endogenous proteins) analysis using mouse liver samples from two circadian time points was used to catalog ERRα-interacting proteins on chromatin. The genomic crosstalk between ERRα and its identified cofacto

SUBMITTER: Scholtes C 

PROVIDER: S-EPMC10990974 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature