Modulation of Pyruvate Export and Extracellular Pyruvate Concentration in Primary Astrocyte Cultures.

ABSTRACT: Astrocyte-derived pyruvate is considered to have neuroprotective functions. In order to investigate the processes that are involved in astrocytic pyruvate release, we used primary rat astrocyte cultures as model system. Depending on the incubation conditions and medium composition, astrocyte cultures established extracellular steady state pyruvate concentrations in the range between 150 µM and 300 µM. During incubations for up to 2 weeks in DMEM culture medium, the extracellular pyruvate concentration remained almost constant for days, while the extracellular lactate concentration increased continuously during the incubation into the millimolar concentration range as long as glucose was present. In an amino acid-free incubation buffer, glucose-fed astrocytes released pyruvate with an initi

SUBMITTER: Denker N 

PROVIDER: S-EPMC10991036 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature