Efficient synthesis 1,4-cyclohexanedicarboxaldehyde by an engineered alcohol oxidase.

ABSTRACT: In this study, we selected and engineered a flavin adenine dinucleotide (FAD)-dependent alcohol oxidase (AOX) to produce 1,4-cyclohexanedicarboxaldehyde (CHDA), an initial raw material for spiral compounds, from 1,4-cyclohexanedimethanol (CHDM). First, the structure of alcohol oxidase from Arthrobacter cholorphenolicus (AcCO) was analyzed, and the mechanism of AcCO-catalyzed primary alcohol oxidation was elucidated, demonstrating that the energy barrier of the hydride (H^-) transfer (13.4 kcal·mol^-1 and 20.4 kcal·mol^-1) decreases the catalytic efficiency of the primary alcohol oxidation reaction. Therefore, we designed a protein engineering strategy to adjust the catalytically active conformation to shorten the distance of hydride (H^-) transfer an

SUBMITTER: Cheng Y 

PROVIDER: S-EPMC10991250 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature