A novel thermophilic chitinase directly mined from the marine metagenome using the deep learning tool Preoptem.

ABSTRACT: Chitin is abundant in nature and its degradation products are highly valuable for numerous applications. Thermophilic chitinases are increasingly appreciated for their capacity to biodegrade chitin at high temperatures and prolonged enzyme stability. Here, using deep learning approaches, we developed a prediction tool, Preoptem, to screen thermophilic proteins. A novel thermophilic chitinase, Chi304, was mined directly from the marine metagenome. Chi304 showed maximum activity at 85 ℃, its T_m reached 89.65 ± 0.22℃, and exhibited excellent thermal stability at 80 and 90 °C. Chi304 had both endo- and exo-chitinase activities, and the (GlcNAc)₂ was the main hydrolysis product of chitin-related substrates. The product yields of colloidal chitin degradation reached 97% wit

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC10991277 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature