Dataset Information

Immune evasion in lung metastasis of leiomyosarcoma: upregulation of EPCAM inhibits CD8⁺ T cell infiltration.

ABSTRACT:

Background

Leiomyosarcomas are among the most common histological types of soft tissue sarcoma (STS), with no effective treatment available for advanced patients. Lung metastasis, the most common site of distant metastasis, is the primary prognostic factor. We analysed the immune environment targeting lung metastasis of STS to explore new targets for immunotherapy.

Methods

We analysed the immune environment of primary and lung metastases in 38 patients with STS using immunohistochemistry. Next, we performed gene expression analyses on primary and lung metastatic tissues from six patients with leiomyosarcoma. Using human leiomyosarcoma cell lines, the effects of the identified genes on immune cells were assessed in vitro.

Results

Immunohistochemistry showed a significant decrease in CD8⁺ cells in the lung metastases of leiomyosarcoma. Among the genes upregulated in lung metastases, epithelial cellular adhesion molecule (EPCAM) showed the strongest negative correlation with the number of CD8⁺ cells. Transwell assay results showed that the migration of CD8⁺ T cells was significantly increased in the conditioned media obtained after inhibition or knock down of EPCAM.

Conclusions

EPCAM was upregulated in lung metastases of leiomyosarcoma, suggesting inhibition of CD8⁺ T cell migration. Our findings suggest that EPCAM could serve as a potential novel therapeutic target for leiomyosarcoma.

SUBMITTER: Kanahori M

PROVIDER: S-EPMC10991329 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature

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Publications

Immune evasion in lung metastasis of leiomyosarcoma: upregulation of EPCAM inhibits CD8<sup>+</sup> T cell infiltration.

Kanahori Masaya M Shimada Eijiro E Matsumoto Yoshihiro Y Endo Makoto M Fujiwara Toshifumi T Nabeshima Akira A Hirose Takeshi T Kawaguchi Kengo K Oyama Ryunosuke R Oda Yoshinao Y Nakashima Yasuharu Y

British journal of cancer 20240130 7

<h4>Background</h4>Leiomyosarcomas are among the most common histological types of soft tissue sarcoma (STS), with no effective treatment available for advanced patients. Lung metastasis, the most common site of distant metastasis, is the primary prognostic factor. We analysed the immune environment targeting lung metastasis of STS to explore new targets for immunotherapy.<h4>Methods</h4>We analysed the immune environment of primary and lung metastases in 38 patients with STS using immunohistoch ...[more]

PMID: 38291183

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Dataset Information

Immune evasion in lung metastasis of leiomyosarcoma: upregulation of EPCAM inhibits CD8⁺ T cell infiltration.

Background

Methods

Results

Conclusions

Publications

Immune evasion in lung metastasis of leiomyosarcoma: upregulation of EPCAM inhibits CD8<sup>+</sup> T cell infiltration.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

Immune evasion in lung metastasis of leiomyosarcoma: upregulation of EPCAM inhibits CD8+ T cell infiltration.

Background

Methods

Results

Conclusions

Publications

Immune evasion in lung metastasis of leiomyosarcoma: upregulation of EPCAM inhibits CD8&lt;sup&gt;+&lt;/sup&gt; T cell infiltration.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Immune evasion in lung metastasis of leiomyosarcoma: upregulation of EPCAM inhibits CD8⁺ T cell infiltration.

Immune evasion in lung metastasis of leiomyosarcoma: upregulation of EPCAM inhibits CD8<sup>+</sup> T cell infiltration.