RPA-CRISPR/Cas12a assay for the diagnosis of bovine Anaplasma marginale infection.

ABSTRACT: Anaplasma marginale infection is one of the most common tick-borne diseases, causing a substantial loss in the beef and dairy production industries. Once infected, the pathogen remains in the cattle for life, allowing the parasites to spread to healthy animals. Since clinical manifestations of anaplasmosis occur late in the disease, a sensitive, accurate, and affordable pathogen identification is crucial in preventing and controlling the infection. To this end, we developed an RPA-CRISPR/Cas12a assay specific to A. marginale infection in bovines targeting the msp4 gene. Our assay is performed at one moderately high temperature, producing fluorescent signals or positive readout of a lateral flow dipstick, which is as sensitive as conventional PCR-based DNA amplification. This RPA-CRISPR/Cas

SUBMITTER: Sutipatanasomboon A 

PROVIDER: S-EPMC10991388 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature