ReLo is a simple and rapid colocalization assay to identify and characterize direct protein-protein interactions.

ABSTRACT: The characterization of protein-protein interactions (PPIs) is fundamental to the understanding of biochemical processes. Many methods have been established to identify and study direct PPIs; however, screening and investigating PPIs involving large or poorly soluble proteins remains challenging. Here, we introduce ReLo, a simple, rapid, and versatile cell culture-based method for detecting and investigating interactions in a cellular context. Our experiments demonstrate that ReLo specifically detects direct binary PPIs. Furthermore, we show that ReLo bridging experiments can also be used to determine the binding topology of subunits within multiprotein complexes. In addition, ReLo facilitates the identification of protein domains that mediate complex formation, allows screening for interf

SUBMITTER: Salgania HK 

PROVIDER: S-EPMC10991417 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature