Differential immune microenvironmental features of microsatellite-unstable colorectal cancers according to Fusobacterium nucleatum status.

ABSTRACT: It has been suggested that Fusobacterium nucleatum (Fn) may differentially impact tumor immune responses according to microsatellite instability (MSI) status in colorectal cancers (CRCs). We aimed to reveal the detailed relationship between intratumoral Fn and immune microenvironmental features in MSI-high CRCs. A total of 126 MSI-high CRCs were subjected to analyses for intratumoral Fn DNA load using quantitative PCR and for densities of tumor-infiltrating immune cells, including CD3⁺ T cells, CD4⁺ T cells, CD8⁺ T cells, FoxP3⁺ T cells, CD68⁺ macrophages, CD163⁺ macrophages, and CD177⁺ neutrophils, at invasive margin (IM) and center of tumor (CT) areas using computational image analysis of immunohistochemistry. B

SUBMITTER: Lee JA 

PROVIDER: S-EPMC10991456 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature