Increased paclitaxel recovery from Taxus baccata vascular stem cells using novel in situ product recovery approaches.

ABSTRACT: In this study, several approaches were tested to optimise the production and recovery of the widely used anticancer drug Taxol^® (paclitaxel) from culturable vascular stem cells (VSCs) of Taxus baccata, which is currently used as a successful cell line for paclitaxel production. An in situ product recovery (ISPR) technique was employed, which involved combining three commercial macro-porous resin beads (HP-20, XAD7HP and HP-2MG) with batch and semi-continuous cultivations of the T. baccata VSCs after adding methyl jasmonate (Me-JA) as an elicitor. The optimal resin combination resulted in 234 ± 23 mg of paclitaxel per kg of fresh-weight cells, indicating a 13-fold improved yield compared to the control (with no resins) in batch cultivation. This resin treatment was further studie

SUBMITTER: Santoyo-Garcia JH 

PROVIDER: S-EPMC10991628 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature