Genetic engineering and raising temperature enhance recombinant protein production with the cdna1 promoter in Trichoderma reesei.

ABSTRACT: The fungus Trichoderma reesei is a powerful host for secreted production of proteins. The promoter of cdna1 gene, which encodes a small basic protein of unknown function and high expression, is commonly used for constitutive protein production in T. reesei. Nevertheless, the production level of proteins driven by this promoter still needs to be improved. Here, we identified that the region 600- to 700-bp upstream of the start codon is critical for the efficiency of the cdna1 promoter. Increasing the copy number of this region to three improved the production of a heterologous β-mannanase by 37.5%. Screening of several stressful conditions revealed that the cdna1 promoter is heat inducible. Cultivation at 37 °C significantly enhanced the production of β-mannanase as well as a polygalacturon

SUBMITTER: Jiang S 

PROVIDER: S-EPMC10991654 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature