Associations of reproductive breast cancer risk factors with expression of stem cell markers in benign breast tissue.

ABSTRACT:

Background

We investigated the associations of reproductive factors known to influence breast cancer risk with the expression of breast stem cell markers CD44, CD24, and ALDH1A1 in benign breast biopsy samples.

Methods

We included 439 cancer-free women with biopsy-confirmed benign breast disease within the Nurses' Health Study (NHS) and NHSII. The data on reproductive and other breast cancer risk factors were obtained from biennial questionnaires. Immunohistochemistry (IHC) was performed on tissue microarrays. For each core, the IHC expression was assessed using a semi-automated platform and expressed as % of cells that stained positive for a specific marker out of the total cell count. Generalized linear regression was used to examine the associations of reproductive factor

SUBMITTER: Yaghjyan L 

PROVIDER: S-EPMC10991780 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature