Comparative analysis of basal and etoposide-induced alterations in gene expression by DNA-PKcs kinase activity.

ABSTRACT: Background: Maintenance of the genome is essential for cell survival, and impairment of the DNA damage response is associated with multiple pathologies including cancer and neurological abnormalities. DNA-PKcs is a DNA repair protein and a core component of the classical nonhomologous end-joining pathway, but it also has roles in modulating gene expression and thus, the overall cellular response to DNA damage. Methods: Using cells producing either wild-type (WT) or kinase-inactive (KR) DNA-PKcs, we assessed global alterations in gene expression in the absence or presence of DNA damage. We evaluated differential gene expression in untreated cells and observed differences in genes associated with cellular adhesion, cell cycle regulation, and inflammation-related pathways. Follo

SUBMITTER: Ali SI 

PROVIDER: S-EPMC10991847 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature