Combinatorial strategies for production improvement of anti-tuberculosis antibiotics ilamycins E₁/E₂ from deep sea-derived Streptomyces atratus SCSIO ZH16 ΔilaR.

ABSTRACT: Ilamycins E₁/E₂ are novel cyclic heptapeptides from Streptomyces atratus SCSIO ZH16, which have the MIC value of 9.8 nM against Mycobacterium tuberculosis H37Rv. However, the lower fermentative titer of ilamycins E₁/E₂ cut off further development for novel anti-TB lead drugs. In order to break the obstacle, the combinatorial strategy of medium optimization, fermentative parameters optimization, exogenous addition of metal ions, precursors, and surfactants was developed to promoted the production of ilamycins E₁/E₂. Addition of 1 mM ZnCl₂ at 0 h, 1 g/L tyrosine at 96 h, and 2 g/L shikimic acid at 48 h increased the production of ilamycins E₁/E₂ from 13.51 to 762.50 ± 23.15, 721.39 ± 19.13, and

SUBMITTER: Zhu Y 

PROVIDER: S-EPMC10992044 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature