Packaging cells based on inducible gene amplification for the production of adeno-associated virus vectors.

ABSTRACT: Although vectors based on adeno-associated virus (AAV) offer several unique advantages, their usage has been hampered by the difficulties encountered in vector production. In this report, we describe a new AAV packaging system based on inducible amplification of integrated helper and vector constructs containing the simian virus 40 (SV40) replication origin. The packaging and producer cell lines developed express SV40 T antigen under the control of the reverse tetracycline transactivator system, which allows inducible amplification of chromosomal loci linked to the SV40 origin. Culturing these cells in the presence of doxycycline followed by adenovirus infection resulted in helper and vector gene amplification as well as higher vector titers. Clonal producer cell lines generated vector tit

SUBMITTER: Inoue N 

PROVIDER: S-EPMC109922 | biostudies-literature | 1998 Sep

REPOSITORIES: biostudies-literature