Constructing Escherichia coli co-display systems for biodegradation of polyethylene terephthalate.

ABSTRACT:

Background

The accumulation of fast-growing polyethylene terephthalate (PET) wastes has posed numerous threats to the environments and human health. Enzymatic degradation of PET is a promising approach for PET waste treatment. Currently, the efficiency of various PET biodegradation systems requires further improvements.

Results

In this work, we engineered whole cell systems with co-display of strong adhesive proteins and the most active PETase for PET biodegradation in E. coli cells. Adhesive proteins of cp52k and mfp-3 and Fast-PETase were simultaneously displayed on the surfaces of E. coli cells, and the resulting cells displaying mfp-3 showed 50% increase of adhesion ability compared to those without adhesive proteins. Consequently, the degradation rate of E. coli cells c

SUBMITTER: Hu J 

PROVIDER: S-EPMC10992762 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature