Optimization of cultivation medium and cyclic fed-batch fermentation strategy for enhanced polyhydroxyalkanoate production by Bacillus thuringiensis using a glucose-rich hydrolyzate.

ABSTRACT: The accumulation of petrochemical plastic waste is detrimental to the environment. Polyhydroxyalkanoates (PHAs) are bacterial-derived polymers utilized for the production of bioplastics. PHA-plastics exhibit mechanical and thermal properties similar to conventional plastics. However, high production cost and obtaining high PHA yield and productivity impedes the widespread use of bioplastics. This study demonstrates the concept of cyclic fed-batch fermentation (CFBF) for enhanced PHA productivity by Bacillus thuringiensis using a glucose-rich hydrolyzate as the sole carbon source. The statistically optimized fermentation conditions used to obtain high cell density biomass (OD₆₀₀ of 2.4175) were: 8.77 g L^-1 yeast extract; 66.63% hydrolyzate (v/v); a fermentation pH of 7

SUBMITTER: Singh S 

PROVIDER: S-EPMC10992944 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature