Exploring Neuronal Differentiation Profiles in SH-SY5Y Cells through Magnetic Levitation Analysis.

ABSTRACT: Magnetic levitation (MagLev) is a powerful and versatile technique that can sort objects based on their density differences. This paper reports the sorting of SH-SY5Y cells for neuronal differentiation by the MagLev technique. Herein, SH-SY5Y cells were differentiated with retinoic acid (RA) and brain-derived neurotrophic factor (BDNF). Neuronal differentiation was confirmed by neurite extension measurement and the immunostaining assay. Neurites reached the maximum length on day 9 after sequential treatment with RA-BDNF. Neuronal marker expression of un-/differentiated cells was investigated by β-III tubulin and neuronal nuclei (NeuN) and differentiated cells exhibited a higher fluorescence intensity compared to un-/differentiated cells. MagLev results revealed that the density of differen

SUBMITTER: Bilginer Kartal R 

PROVIDER: S-EPMC10993277 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature