Stigmasterol alleviates neuropathic pain by reducing Schwann cell-macrophage cascade in DRG by modulating IL-34/CSF1R.

ABSTRACT:

Aims

This study aimed to investigate the potential therapeutic applications of stigmasterol for treating neuropathic pain.

Methods

Related mechanisms were investigated by DRG single-cell sequencing analysis and the use of specific inhibitors in cellular experiments. In animal experiments, 32 male Sprague-Dawley rats were randomly divided into the sham operation group, CCI group, ibuprofen group, and stigmasterol group. We performed behavioral tests, ELISA, H&E staining and immunohistochemistry, and western blotting.

Results

Cell communication analysis by single-cell sequencing reveals that after peripheral nerve injury, Schwann cells secrete IL-34 to act on CSF1R in macrophages. After peripheral nerve injury, the mRNA expression levels of CSF1R pathway and NLRP3 infla

SUBMITTER: Si W 

PROVIDER: S-EPMC10993342 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature