Novel insights into phage biology of the pathogen Clostridioides difficile based on the active virome.

ABSTRACT: The global pathogen Clostridioides difficile is a well-studied organism, and researchers work on unraveling its fundamental virulence mechanisms and biology. Prophages have been demonstrated to influence C. difficile toxin expression and contribute to the distribution of advantageous genes. All these underline the importance of prophages in C. difficile virulence. Although several C. difficile prophages were sequenced and characterized, investigations on the entire active virome of a strain are still missing. Phages were mainly isolated after mitomycin C-induction, which does not resemble a natural stressor for C. difficile. We examined active prophages from different C. difficile strains after cultivation in the absence of mitomycin C by sequencin

SUBMITTER: Schuler MA 

PROVIDER: S-EPMC10993401 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature