Global and single-nucleotide resolution detection of 7-methylguanosine in RNA.

ABSTRACT: RNA modifications, including N-7-methylguanosine (m⁷G), are pivotal in governing RNA stability and gene expression regulation. The accurate detection of internal m⁷G modifications is of paramount significance, given recent associations between altered m⁷G deposition and elevated expression of the methyltransferase METTL1 in various human cancers. The development of robust m⁷G detection techniques has posed a significant challenge in the field of epitranscriptomics. In this study, we introduce two methodologies for the global and accurate identification of m⁷G modifications in human RNA. We introduce borohydride reduction sequencing (Bo-Seq), which provides base resolution mapping of m⁷G modifications. Bo-Seq achieves

SUBMITTER: D'Ambrosi S 

PROVIDER: S-EPMC10993922 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature