Genome-resolved metagenomics identifies novel active microbes in biogeochemical cycling within methanol-enriched soil.

ABSTRACT: Metagenome assembled genomes (MAGs), generated from sequenced ¹³C-labelled DNA from ¹³C-methanol enriched soils, were binned using an ensemble approach. This method produced a significantly larger number of higher-quality MAGs compared to direct binning approaches. These MAGs represent both the primary methanol utilizers and the secondary utilizers labelled via cross-feeding and predation on the labelled methylotrophs, including numerous uncultivated taxa. Analysis of these MAGs enabled the identification of multiple metabolic pathways within these active taxa that have climatic relevance relating to nitrogen, sulfur and trace gas metabolism. This includes denitrification, dissimilatory nitrate reduction to ammonium, ammonia oxidation and metabolism of organic sulfur

SUBMITTER: Macey MC 

PROVIDER: S-EPMC10994693 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature