Manufacturing of primary CAR-NK cells in an automated system for the treatment of acute myeloid leukemia.

ABSTRACT: Acute myeloid leukemia (AML) still constitutes a dreadful disease with limited therapeutic options. Chimeric antigen receptor (CAR)-modified T cells struggle to target AML partly due to a lack of true AML-exclusive antigens and heterogeneity of the disease. Natural killer (NK) cells possess a high intrinsic killing capacity against AML and might be well suited for the treatment of this disease. However, the generation of primary CAR-NK cells can be difficult and time consuming. Therefore, robust systems for the generation of high numbers of CAR-NK cells under GMP conditions are required. Here we report on the automated generation of high numbers of primary CD33-targeting CAR-NK cells using the CliniMACS Prodigy^® platform. Automated-produced CD33-CAR-NK cells showed similar pheno

SUBMITTER: Albinger N 

PROVIDER: S-EPMC10994833 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature