Aberrant splicing of Ca_V1.2 calcium channel induced by decreased Rbfox1 enhances arterial constriction during diabetic hyperglycemia.

ABSTRACT: Diabetic hyperglycemia induces dysfunctions of arterial smooth muscle, leading to diabetic vascular complications. The Ca_V1.2 calcium channel is one primary pathway for Ca²⁺ influx, which initiates vasoconstriction. However, the long-term regulation mechanism(s) for vascular Ca_V1.2 functions under hyperglycemic condition remains unknown. Here, Sprague-Dawley rats fed with high-fat diet in combination with low dose streptozotocin and Goto-Kakizaki (GK) rats were used as diabetic models. Isolated mesenteric arteries (MAs) and vascular smooth muscle cells (VSMCs) from rat models were used to assess K⁺-induced arterial constriction and Ca_V1.2 channel functions using vascular myograph and whole-cell patch clamp, respectively. K⁺-

SUBMITTER: Hou W 

PROVIDER: S-EPMC10995029 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature