TRPV4-dependent Ca²⁺ influx determines cholesterol dynamics at the plasma membrane.

ABSTRACT: The activities of the transient receptor potential vanilloid 4 (TRPV4), a Ca²⁺-permeable nonselective cation channel, are controlled by its surrounding membrane lipids (e.g., cholesterol, phosphoinositides). The transmembrane region of TRPV4 contains a cholesterol recognition amino acid consensus (CRAC) motif and its inverted (CARC) motif located in the plasmalemmal cytosolic leaflet. TRPV4 localizes in caveolae, a bulb-shaped cholesterol-rich domain at the plasma membrane. Here, we visualized the spatiotemporal interactions between TRPV4 and cholesterol at the plasma membrane in living cells by dual-color single-molecule imaging using total internal reflection fluorescence microscopy. To this aim, we labeled cholesterol at the cytosolic leaflets of the plasma membrane using a c

SUBMITTER: Kuwashima Y 

PROVIDER: S-EPMC10995426 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature