Pseudomonas hefeiensis sp. nov., isolated from the rhizosphere of multiple cash crops in China.

ABSTRACT: Three bacterial strains, FP250^T, FP821, and FP53, were isolated from the rhizosphere soil of oilseed rape, licorice, and habanero pepper in Anhui Province, Xinjiang Uygur Autonomous Region, and Jiangsu Province, PR China, respectively. All strains were shown to grow at 4-37 °C and pH 6.0-9.0, and in the presence of 0-4.0 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences or housekeeping genes (16S rRNA, gyrB, rpoB, and rpoD) and phylogenomic analysis showed that strains FP250^T, FP821, and FP53 belong to the genus Pseudomonas, and are closely related to Pseudomonas kilonensis DSM 13647^T, Pseudomonas brassicacearum JCM 11938^T, Pseudomonas viciae 11K1^T, and Pseudomonas thivervalensis DSM 13194^T. The DNA G+C content of strain FP205^T was 59.8 mol%. The average nucleotide identity and digital DNA-DNA hybridization values of strain FP205^T with the most closely related strain were 93.2 % and 51.4 %, respectively, which is well below the threshold for species differentiation. Strain FP205^T contained summed feature 3 (C_16 : 1  ω6c and/or C_16 : 1  ω7c), summed feature 8 (C_18 : 1  ω7c and/or C_18 : 1  ω6c) as major fatty acids, and diphosphatidylglycerol along with phosphatidylethanolamine and aminophospholipid as major polar lipids. The predominant isoprenoid quinone was ubiquinone-9. Based on these phenotypic, phylogenetic, and chemotaxonomic results, strain FP205^T represents a novel species of the genus Pseudomonas, for which the name Pseudomonas hefeiensis sp. nov. is proposed. The type strain is FP205^T (=ACCC 62447^T=JCM 35687^T).

SUBMITTER: Liao K 

PROVIDER: S-EPMC10995727 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature