Cell-Surface Binding of DNA Nanostructures for Enhanced Intracellular and Intranuclear Delivery.

ABSTRACT: DNA nanostructures (DNs) have found increasing use in biosensing, drug delivery, and therapeutics because of their customizable assembly, size and shape control, and facile functionalization. However, their limited cellular uptake and nuclear delivery have hindered their effectiveness in these applications. Here, we demonstrate the potential of applying cell-surface binding as a general strategy to enable rapid enhancement of intracellular and intranuclear delivery of DNs. By targeting the plasma membrane via cholesterol anchors or the cell-surface glycocalyx using click chemistry, we observe a significant 2 to 8-fold increase in the cellular uptake of three distinct types of DNs that include nanospheres, nanorods, and nanotiles, within a short time frame of half an hour. Several factors a

SUBMITTER: Wang W 

PROVIDER: S-EPMC10995898 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature