Increase of Phosphoprotein Expressions in Amotosalen/UVA-Treated Platelet Concentrates.

ABSTRACT:

Background

Pathogen inactivation treatment (PIT) has been shown to alter platelet function, phenotype, morphology and to induce a faster aging of platelet concentrates (PCs). Key pieces of information are still missing to understand the impacts of PITs at the cellular level.

Objectives

This study investigated the impact of amotosalen/UVA on PCs, from a post-translational modifications (PTM) point of view. Phosphoproteomic analyses were conducted on resting platelets, right after the amotosalen/UVA treatment and compared with untreated PCs.

Method

A two-arm study setting was carried out to compare PIT (amotosalen/UVA) to untreated PCs, on day 1 post-donation. Based on a pool-and-split approach, 12 PCs were split into two groups (treated and untreated). Quantitative pho

SUBMITTER: Muret C 

PROVIDER: S-EPMC10996061 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature