An adapted protocol to derive microglia from stem cells and its application in the study of CSF1R-related disorders.

ABSTRACT:

Background

Induced pluripotent stem cell-derived microglia (iMGL) represent an excellent tool in studying microglial function in health and disease. Yet, since differentiation and survival of iMGL are highly reliant on colony-stimulating factor 1 receptor (CSF1R) signaling, it is difficult to use iMGL to study microglial dysfunction associated with pathogenic defects in CSF1R.

Methods

Serial modifications to an existing iMGL protocol were made, including but not limited to changes in growth factor combination to drive microglial differentiation, until successful derivation of microglia-like cells from an adult-onset leukoencephalopathy with axonal spheroids and pigmented glia (ALSP) patient carrying a c.2350G > A (p.V784M) CSF1R variant. Using healthy control lines, the qual

SUBMITTER: Dorion MF 

PROVIDER: S-EPMC10996091 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature