Identification of ferroptosis related genes and pathways in prostate cancer cells under erastin exposure.

ABSTRACT:

Background

Few studies are focusing on the mechanism of erastin acts on prostate cancer (PCa) cells, and essential ferroptosis-related genes (FRGs) that can be PCa therapeutic targets are rarely known.

Methods

In this study, in vitro assays were performed and RNA-sequencing was used to measure the expression of differentially expressed genes (DEGs) in erastin-induced PCa cells. A series of bioinformatic analyses were applied to analyze the pathways and DEGs.

Results

Erastin inhibited the expression of SLC7A11 and cell survivability in LNCaP and PC3 cells. After treatment with erastin, the concentrations of malondialdehyde (MDA) and Fe²⁺ significantly increased, whereas the glutathione (GSH) and the oxidized glutathione (GSSG) significantly decreased in both

SUBMITTER: Wu F 

PROVIDER: S-EPMC10996193 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature