A macrophage-cell model of HIV latency reveals the unusual importance of the bromodomain axis.

ABSTRACT:

Background

Although macrophages are now recognized as an essential part of the HIV latent reservoir, whether and how viral latency is established and reactivated in these cell types is poorly understood. To understand the fundamental mechanisms of viral latency in macrophages, there is an urgent need to develop latency models amenable to genetic manipulations and screening for appropriate latency-reversing agents (LRAs). Given that differentiated THP-1 cells resemble monocyte-derived macrophages in HIV replication mechanisms, we set out to establish a macrophage cell model for HIV latency using THP-1 cells.

Methods

We created single-cell clones of THP-1 cells infected with a single copy of the dual-labeled HIV_GKO in which a codon switched eGFP (csGFP) is under the

SUBMITTER: Kisaka JK 

PROVIDER: S-EPMC10996205 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature