Trx4, a novel thioredoxin protein, is important for Toxoplasma gondii fitness.

ABSTRACT:

Background

To successfully replicate within the host cell, Toxoplasma gondii employs several mechanisms to overcome the host cell defenses and mitigate the harmful effects of the free radicals resulting from its own metabolic processes using effectors such as thioredoxin proteins. In this study, we characterize the location and functions of a newly identified thioredoxin in T. gondii, which was named Trx4.

Methods

We characterized the functional role of Trx4 in T. gondii Type I RH and Type II Pru strains by gene knockout and studied its subcellular localization by endogenous protein HA tagging using CRISPR-Cas9 gene editing. The enzyme-catalyzed proximity labeling technique, the TurboID system, was employed to identify the proteins in proximity to Trx4.

Results

Trx4 w

SUBMITTER: Zhang ZW 

PROVIDER: S-EPMC10996207 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature