Single-cell analysis of mesenchymal cells in permeable neural vasculature reveals novel diverse subpopulations of fibroblasts.

ABSTRACT:

Background

In the choroid plexus and pituitary gland, vasculature is known to have a permeable, fenestrated phenotype which allows for the free passage of molecules in contrast to the blood brain barrier observed in the rest of the CNS. The endothelium of these compartments, along with secretory, neural-lineage cells (choroid epithelium and pituitary endocrine cells) have been studied in detail, but less attention has been given to the perivascular mesenchymal cells of these compartments.

Methods

The Hic1^CreERT2 Rosa26^LSL-TdTomato mouse model was used in conjunction with a Pdgfra^H2B-EGFP mouse model to examine mesenchymal cells, which can be subdivided into Pdgfra⁺ fibroblasts and Pdgfra^- pericytes within the choroid ple

SUBMITTER: Bastedo WE 

PROVIDER: S-EPMC10996213 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature