Intestinal Piezo1 aggravates intestinal barrier dysfunction during sepsis by mediating Ca²⁺ influx.

ABSTRACT:

Introduction

Intestinal barrier dysfunction is a pivotal factor in sepsis progression. The mechanosensitive ion channel Piezo1 is associated with barrier function; however, its role in sepsis-induced intestinal barrier dysfunction remains poorly understood.

Methods

The application of cecal ligation and puncture (CLP) modeling was performed on both mice of the wild-type (WT) variety and those with Villin-Piezo1^flox/flox genetic makeup to assess the barrier function using in vivo FITC-dextran permeability measurements and immunofluorescence microscopy analysis of tight junctions (TJs) and apoptosis levels. In vitro, Caco-2 monolayers were subjected to TNF-α incubation. Moreover, to modulate Piezo1 activation, GsMTx4 was applied to inhibit Piezo1 activation. The barr

SUBMITTER: Yan Z 

PROVIDER: S-EPMC10996241 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature