Analysis of the limited <i>M. tuberculosis</i> accessory genome reveals potential pitfalls of pan-genome analysis approaches.

ABSTRACT: Pan-genome analysis is a fundamental tool for studying bacterial genome evolution; however, the variety of methods used to define and measure the pan-genome poses challenges to the interpretation and reliability of results. To quantify sources of bias and error related to common pan-genome analysis approaches, we evaluated different approaches applied to curated collection of 151 Mycobacterium tuberculosis ( Mtb ) isolates. Mtb is characterized by its clonal evolution, absence of horizontal gene transfer, and limited accessory genome, making it an ideal test case for this study. Using a state-of-the-art graph-genome approach, we found that a majority of the structural variation observed in Mtb originates from rearrangement, deletion, and duplication of redundant

SUBMITTER: Marin MG 

PROVIDER: S-EPMC10996470 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature