Micron-resolution fiber mapping in histology independent of sample preparation.

ABSTRACT: Mapping the brain's fiber network is crucial for understanding its function and malfunction, but resolving nerve trajectories over large fields of view is challenging. Electron microscopy only studies small brain volumes, diffusion magnetic resonance imaging (dMRI) has limited spatial resolution, and polarization microscopy provides unidirectional orientations in birefringence-preserving tissues. Scattered light imaging (SLI) has previously enabled micron-resolution mapping of multi-directional fibers in unstained brain cryo-sections. Here, we show that using a highly sensitive setup, computational SLI (ComSLI) can map fiber networks in histology independent of sample preparation, also in formalin-fixed paraffin-embedded (FFPE) tissues including whole human brain sections. We showcase this

SUBMITTER: Georgiadis M 

PROVIDER: S-EPMC10996646 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature