Loss of LasR function leads to decreased repression of <i>Pseudomonas aeruginosa</i> PhoB activity at physiological phosphate concentrations.

ABSTRACT: While the Pseudomonas aeruginosa LasR transcription factor plays a role in quorum sensing (QS) across phylogenetically-distinct lineages, isolates with loss-of-function mutations in lasR (LasR- strains) are commonly found in diverse settings including infections where they are associated with worse clinical outcomes. In LasR- strains, the transcription factor RhlR, which is controlled by LasR, can be alternately activated in low inorganic phosphate (Pi) concentrations via the two-component system PhoR-PhoB. Here, we demonstrate a new link between LasR and PhoB in which the absence of LasR increases PhoB activity at physiological Pi concentrations and raises the Pi concentration necessary for PhoB inhibition. PhoB activity was also less repressed by Pi in mutants lacking diffe

SUBMITTER: Conaway A 

PROVIDER: S-EPMC10996656 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature