Regenerative human liver organoids (HLOs) in a pillar/perfusion plate for hepatotoxicity assays.

ABSTRACT: Human liver organoids (HLOs) differentiated from embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs), and adult stem cells (ASCs) can recapitulate structure and function of human fetal liver tissues, thus, considered as a promising tissue model for liver diseases and predictive compound screening. Nonetheless, there are still several technical challenges to adopt HLOs in the drug discovery process, which include relatively long-term cell differentiation with multiple culture media (3 - 4 weeks) leading to batch-to-batch variation, short-term hepatic function after maturation (3 - 5 days), low assay throughput due to Matrigel dissociation and HLO transfer to a microtiter well plate, and insufficient maturity as compared to primary hepatocytes. To address these issues, expand

SUBMITTER: Shrestha S 

PROVIDER: S-EPMC10996672 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature