Dysregulation of Autophagy Occurs During Congenital Cataract Development in βA3ΔG91 Mice.

ABSTRACT:

Purpose

To examine lens phenotypic characteristics in βA3ΔG91 mice and determine if βA3ΔG91 affects autophagy in the lens.

Methods

We generated a βA3ΔG91 mouse model using CRISPR/Cas9 methodology. Comparative phenotypic and biochemical characterizations of lenses from postnatal day 0 (P0), P15, and 1-month-old βA3ΔG91 and wild-type (WT) mice were performed. The methodologies used included non-invasive slit-lamp examination, reverse transcription-quantitative polymerase chain reaction (RT-qPCR), western blot, and immunohistochemical (IHC) analyses to determine the levels of autophagy-related genes and proteins. Transmission electron microscopy (TEM) analysis of lenses was performed to assess organelle degradation and the presence of autophagic vesicles. TUNEL staining was use

SUBMITTER: Boateng AK 

PROVIDER: S-EPMC10996937 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature