ERMA (TMEM94) is a P-type ATPase transporter for Mg²⁺ uptake in the endoplasmic reticulum.

ABSTRACT: Intracellular Mg²⁺ (_iMg²⁺) is bound with phosphometabolites, nucleic acids, and proteins in eukaryotes. Little is known about the intracellular compartmentalization and molecular details of Mg²⁺ transport into/from cellular organelles such as the endoplasmic reticulum (ER). We found that the ER is a major _iMg²⁺ compartment refilled by a largely uncharacterized ER-localized protein, TMEM94. Conventional and AlphaFold2 predictions suggest that ERMA (TMEM94) is a multi-pass transmembrane protein with large cytosolic headpiece actuator, nucleotide, and phosphorylation domains, analogous to P-type ATPases. However, ERMA uniquely combines a P-type ATPase domain and a GMN motif for _ERMg²⁺ uptake. Experiments r

SUBMITTER: Vishnu N 

PROVIDER: S-EPMC10997467 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature