FAST, a method based on split-GFP for the detection in solution of proteins synthesized in cell-free expression systems.

ABSTRACT: Cell-free protein synthesis (CFPS) systems offer a versatile platform for a wide range of applications. However, the traditional methods for detecting proteins synthesized in CFPS, such as radioactive labeling, fluorescent tagging, or electrophoretic separation, may be impractical, due to environmental hazards, high costs, technical complexity, and time consuming procedures. These limitations underscore the need for new approaches that streamline the detection process, facilitating broader application of CFPS. By harnessing the reassembly capabilities of two GFP fragments-specifically, the GFP1-10 and GFP11 fragments-we have crafted a method that simplifies the detection of in vitro synthesized proteins called FAST (Fluorescent Assembly of Split-GFP for Translation Tests). FAST relies on t

SUBMITTER: Pham TD 

PROVIDER: S-EPMC10997616 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature