Catalytic-dependent and -independent roles of TET3 in the regulation of specific genetic programs during neuroectoderm specification.

ABSTRACT: The ten-eleven-translocation family of proteins (TET1/2/3) are epigenetic regulators of gene expression. They regulate genes by promoting DNA demethylation (i.e., catalytic activity) and by partnering with regulatory proteins (i.e., non-catalytic functions). Unlike Tet1 and Tet2, Tet3 is not expressed in mouse embryonic stem cells (ESCs) but is induced upon ESC differentiation. However, the significance of its dual roles in lineage specification is less defined. By generating TET3 catalytic-mutant (Tet3^m/m) and knockout (Tet3^-/-) mouse ESCs and differentiating them to neuroectoderm (NE), we identify distinct catalytic-dependent and independent roles of TET3 in NE specification. We find that the catalytic activity of TET3 is important for activation of neural genes whi

SUBMITTER: Ketchum HC 

PROVIDER: S-EPMC10997653 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature