Rapid and sensitive point-of-care diagnosis of human cytomegalovirus infection using RPA-CRISPR technology.

ABSTRACT:

Background

Human cytomegalovirus (HCMV) is a common herpesvirus that can cause a range of symptoms, from mild conditions such as fevers to severe illnesses like pneumonia. Early and accurate diagnosis of HCMV infection is crucial, particularly for vulnerable populations with limited medical care. However, current diagnostic methods are often expensive, time-consuming, and require skilled technicians.

Materials and methods

We developed an HCMV-RPA-CRISPR diagnosis platform for the rapid and cost-effective detection of HCMV infection. This method utilizes recombinase polymerase amplification (RPA) to amplify the HCMV target gene isothermally without the need for thermal cycling equipment. The platform integrates the CRISPR/Cas12a system, significantly enhancing specificity and

SUBMITTER: Shin K 

PROVIDER: S-EPMC10998215 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature