MiR-380 inhibits the proliferation and invasion of cholangiocarcinoma cells by silencing LIS1.

ABSTRACT:

Background

The objective of this study was to determine the role and regulatory mechanism of miR-380 in cholangiocarcinoma.

Methods

The TargetScan database and a dual-luciferase reporter assay system were used to determine if LIS1 was a target gene of miR-380. The Cell Counting Kit 8 assay, flow cytometry, and Transwell assay were used to detect the effects of miR-380 and LIS1 on the proliferation, S-phase ratio, and invasiveness of HCCC-9810/HuCCT1/QBC939 cells. Western blotting was used to determine the effect of miR-380 on MMP-2/p-AKT. Immunohistochemistry detected the regulatory effect of miR-380 on the expression of MMP-2/p-AKT/LIS1.

Results

Expression of miR-380 in cholangiocarcinoma was decreased but expression of LIS1 was increased. LIS1 was confirmed to be a

SUBMITTER: Wei Z 

PROVIDER: S-EPMC10998336 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature