Single-cell profiling reveals transcriptome dynamics during bovine oocyte growth.

ABSTRACT:

Background

Mammalian follicle development is characterized by extensive changes in morphology, endocrine responsiveness, and function, providing the optimum environment for oocyte growth, development, and resumption of meiosis. In cattle, the first signs of transcription activation in the oocyte are observed in the secondary follicle, later than during mouse and human oogenesis. While many studies have generated extensive datasets characterizing gene expression in bovine oocytes, they are mostly limited to the analysis of fully grown and matured oocytes. The aim of the present study was to apply single-cell RNA sequencing to interrogate the transcriptome of the growing bovine oocyte from the secondary follicle stage through to the mid-antral follicle stage.

Results

Single-ce

SUBMITTER: Latorraca LB 

PROVIDER: S-EPMC10998374 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature