Optimization of fermentation conditions and medium components for chrysomycin a production by Streptomyces sp. 891-B6.

ABSTRACT:

Background

Chrysomycin A (CA) is a promising antibiotic for treatment of Gram-positive bacterial infections and cancers. In order to enhance CA yield, optimization of fermentation conditions and medium components was carried out on strain Streptomyces sp. 891-B6, an UV-induced mutant with improved CA titer compared with its wide-type marine strain 891.

Results

Using one-way experiment, the optimal fermentation conditions for CA production in 1-L shake flask were obtained as follows: 12 days of fermentation time, 5 days of seed age, 5% of inoculum volume ratio, 200 mL of loading volume and 6.5 of initial pH. By response surface methodology, the optimal medium components determined as glucose (39.283 g/L), corn starch (20.662 g/L), soybean meal (15.480 g/L) and CaCO₃

SUBMITTER: Hu Z 

PROVIDER: S-EPMC10998411 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature