A sodium-dependent trehalose transporter contributes to anhydrobiosis in insect cell line, Pv11.

ABSTRACT: Pv11 is the only animal cell line that, when preconditioned with a high concentration of trehalose, can be preserved in the dry state at room temperature for more than one year while retaining the ability to resume proliferation. This extreme desiccation tolerance is referred to as anhydrobiosis. Here, we identified a transporter that contributes to the recovery of Pv11 cells from anhydrobiosis. In general, the solute carrier 5 (SLC5)-type secondary active transporters cotransport Na⁺ and carbohydrates including glucose. The heterologous expression systems showed that the transporter belonging to the SLC5 family, whose expression increases upon rehydration, exhibits Na⁺-dependent trehalose transport activity. Therefore, we named it STRT1 (sodium-ion trehalose transpor

SUBMITTER: Mizutani K 

PROVIDER: S-EPMC10998604 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature