Systematic HOIP interactome profiling reveals critical roles of linear ubiquitination in tissue homeostasis.

ABSTRACT: Linear ubiquitination catalyzed by HOIL-1-interacting protein (HOIP), the key component of the linear ubiquitination assembly complex, plays fundamental roles in tissue homeostasis by executing domain-specific regulatory functions. However, a proteome-wide analysis of the domain-specific interactome of HOIP across tissues is lacking. Here, we present a comprehensive mass spectrometry-based interactome profiling of four HOIP domains in nine mouse tissues. The interaction dataset provides a high-quality HOIP interactome resource with an average of approximately 90 interactors for each bait per tissue. HOIP tissue interactome presents a systematic understanding of linear ubiquitination functions in each tissue and also shows associations of tissue functions to genetic diseases. HOIP domain in

SUBMITTER: Fu Y 

PROVIDER: S-EPMC10998861 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature