Inhibition of acyl-CoA binding protein (ACBP) by means of a GABA_ARγ2-derived peptide.

ABSTRACT: Acyl-CoA binding protein (ACBP) encoded by diazepam binding inhibitor (DBI) is an extracellular inhibitor of autophagy acting on the gamma-aminobutyric acid A receptor (GABA_AR) γ2 subunit (GABA_ARγ2). Here, we show that lipoanabolic diets cause an upregulation of GABA_ARγ2 protein in liver hepatocytes but not in other major organs. ACBP/DBI inhibition by systemically injected antibodies has been demonstrated to mediate anorexigenic and organ-protective, autophagy-dependent effects. Here, we set out to develop a new strategy for developing ACBP/DBI antagonists. For this, we built a molecular model of the interaction of ACBP/DBI with peptides derived from GABA_ARγ2. We then validated the interaction between recombinant and native ACBP/DBI protein and

SUBMITTER: Anagnostopoulos G 

PROVIDER: S-EPMC10998878 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature